弘扬茶文化 促进社会主义和谐社会建设

中国是茶的故乡,是世界上茶历史最悠久、茶资源最丰富、茶文化最深厚的国家.茶在中国始终扮演着社会交往的最佳礼物和媒介,是人与人之间友谊的桥梁.茶文化的本质是"和谐文化",它引导人们与自然和谐相处,启示人们在人与人的关系上,要彼此尊重,加强交流,启发人们感悟身心的统一.大力弘扬茶文化精神,是建设和谐文化的一笔重要资源,是构建和谐社会的重要载体.     

克瑞森无核葡萄的主要性状和栽培技术

克瑞森无核为晚熟无核葡萄优良品种。果穗圆锥形，平均重500g；果粒椭圆形，平均重4g，经赤霉素处理后可达6g；果皮红色，果肉黄色，脆硬味甜，口感极佳；10月中下旬成熟，耐贮运，冷藏案件下可存放5个月：抗病能力强于红地球。栽培时宜采用小棚架长梢修剪；合理灌水并严格控制生长后期氮肥的施用；采用化学疏花疏果技术，套袋果适时脱袋、及时采收。注意阴雨天不得进行抹芽、剪梢，以防白腐病侵染，     

中药制剂防治肉鸡肾性传染性支气管炎的实验研究

将某养殖场已确诊为肾性传染性支气管炎的25日龄肉鸡300只，随机分为6组，应用研制的复方中药制剂——禽速康口服液按不同剂量组合进行治疗试验。结果表明，禽速康(3mL／kg)与肾舒宝(40mg／kg)联合疗法，对内鸡肾性传染性支气管炎有较好的疗效，用药3d可控制发病鸡死亡，治愈率达82％。经大群推广试验表明，禽速康与肾舒宝联合防治肉鸡肾性传染性支气管炎，效果确实，防治有效率达93．75％。     

基于Web内嵌Office控件的文档管理平台研究

阐述了基于web内嵌office控件的文档管理平台的基本框架结构,详细介绍了利用web-office控件实现模板的创建和文档的创建的设计原理,以及整个文档管理平台模块的详细功能实现。     

基于EST-SSR和SNP标记的大麦麦芽纯度检测

大麦麦芽作为啤酒酿造的主要原料之一,其纯度决定了麦芽原料的均一性,进而影响加工工艺和啤酒品质。为高效准确地鉴定麦芽纯度,在啤酒企业进行麦芽原料采购和质量监测时提供参考依据。本研究分别利用EST-SSR和SNP标记定性检测了按比例预混的麦芽样品纯度,并利用SNP标记定量检测了4份送检的麦芽盲样纯度。结果表明,EST-SSR标记能定性检测混杂度高于10%的麦芽样品,而SNP标记能够有效鉴定混杂度低至5%的麦芽样品。SNP标记对纯度定量检测的单次抽样的测定值与真实值之间的误差在3%以内。比较发现,本研究所用的两类分子标记均可用于麦芽样品的纯度检测,但基于KASP技术的SNP标记可以满足麦芽纯度的快速定量检测需要。     

Cu~(2+)胁迫对花椰菜种子的萌发及幼苗生理特性的影响

以花椰菜为试材,用不同浓度Cu~(2+)(12.5、25.0、50.0、100.0mg·L-1)处理花椰菜种子,研究Cu~(2+)胁迫对花椰菜种子的萌发及幼苗生理特性的影响。结果表明:随Cu~(2+)浓度增加,花椰菜植物种子的萌发受到抑制;对根的生长发育影响较大,叶片内叶绿素含量和类胡萝卜素含量呈下降趋势;超氧化物歧化酶(SOD)、过氧化物酶(POD)活性总体上升,丙二醛(MDA)及脯氨酸(Pro)含量呈先升后降的趋势。     

观赏植物养护专用缓释肥在3种植物上的应用研究初报

就一种新型观赏植物养护专用缓释肥在醉蝶花、罗汉松、莞香3种观赏植物上的应用效果进行初步探讨。结果表明:缓释肥可促进3种观赏植物的生长,与复合肥相比,该缓释肥具有肥效慢、肥效持续时间长、肥料利用效率高的特点。试验还发现,适量施用缓释肥可促进醉蝶花开花和延长开花期,且该缓释肥覆土施用要明显优于表土施用。     

Characters of the fibroblast-like cells cultured from the mobilized peripheral blood cells

AIM: To explore the characters of fibroblast-like (F-L) cells cultured from granunocyte clony stimunating factor (G-CSF)-mobilized peripheral blood cell (PBC) harvests. METHODS: The adherent cells in the PBC harvests were cultured for 2 week in the mediums of RPMI-1640/L-DMEM/G-CSF or interleukin-3 (IL-3) plus RPMI-1640, the cultured F-L cells were analyzed by flow cytometry (FC). RESULTS: The adherent non-confluent F-L cells obtained from the four groups were similar in their phenotypes: CD33+, CD11c+, CD64+, CD14+, CD45+, HLA-DR+, CD86+, CD34-, CD38-, CD3-, CD19-, CD56-, CD29-, CD44-, CD105-. The F-L cells are similar to monocytes except CD38- and were distinct from dendritic cells (DC) or mesenchymal stem cells (MSC). CONCLUSION: The cultured F-L cells are macrophages rather than DC or MSC. G-CSF, rhIL-3 enhances their numbers.     

乳鼠成骨细胞的培养及鉴定

无菌采取5日龄SD大鼠的颅盖骨,用胰蛋白酶预消化后,漂洗,剪碎,再用Ⅱ型胶原酶消化获得成骨细胞。通过倒置相差显微镜观察,并用碱性磷酸酶和矿化结节染色鉴定,利用微量滴定（MTT）法测定一个培养周期的成骨细胞活性分布,探讨一种经济、高效的原代成骨细胞培养方法。倒置相差显微镜形态观察结果显示,未贴壁的细胞呈圆形,贴壁生长的细胞呈不规则梭形、三角形或多角形,单核,有1-3个核仁;碱性磷酸酶和钙化结节染色均呈阳性（阳性率≥98.06%）;MTT法结合形态学观察结果显示,成骨细胞培养至第10 d时活性最强。证实用改良的二次酶消化法能够在短时间内获得大量成骨细胞,且所培养的细胞具有典型的成骨细胞形态和功能。     

Effects of different chemotherapeutic agents on reversing the acquired resistance to TRAIL gene in DLD1 colon cancer cells

AIM:To evaluate effects of different chemotherapeutic agents on reversing the acquired resistance to TRAIL gene and clarify the involved mechanisms in DLD1-TRAIL/R colon cancer cells. METHODS:Human colon cancer cell line DLD1-TRAIL/R cells that were resistant to TRAIL-expressing adenovector (Ad/gTRAIL) were treated with Ad/gTRAIL combined with different chemotherapeutic agents. Then, the cell viability was measured by MTT method, and apoptotic signaling conditions, including activation of caspase-3 and caspase-8, expression of Bax and Bcl-XL, were measured by Western blotting analysis. RESULTS:In vitro data showed that several chemotherapeutic agents, including 5-fluorouracil (5-FU) and mitomycin c (MMC), overcome the acquired resistance to TRAIL gene in DLD1-TRAIL/R colon cancer cells. The combination of Ad/gTRAIL and 5-FU effectively suppressed tumor growth in vivo in subcutaneous tumors established from DLD1-TRAIL/R cells. Further data showed that treatment with the combination of Ad/gTRAIL and 5-FU or MMC led to enhance the activation of caspase-3. Moreover, MMC but not 5-FU induced overexpression of Bax gene that was sufficient to overcome the resistance to TRAIL gene in DLD1-TRAIL/R cells. CONCLUSION:Chemotherapeutic agents, such as 5-FU and MMC, overcome the acquired resistance to TRAIL gene in DLD1-TRAIL/R cells. The candidate mechanisms for MMC but not 5-FU to overcome this resistance might involve the induction of over-expressed Bax protein in DLD1-TRAIL/R cells.